ABSTRACT
The aimed study is an illustrative investigation on L-glutamic acid production using inexpensive fermentable substrate-fruits of Mimusops elengi Linn. for the establishment of an economical fermentation process to meet the growing demands of Lglutamic acid in the global market at reasonable price. This profitable substrate produces opulent yield of L-glutamic acid under precise solid state fermentation conditions upon inoculation with Arthrobacter globiformis MTCC 4299. The production was tested at varying physico-chemical parameters like pH, temperature, time, urea, biotin and penicillin concentration. The highest yield obtained was 87.5 g of L-glutamic acid per 100g of sugars consumed at pH 5.0, temperature 300c upon 3d of incubation with urea, biotin and Penicillin optimum concentration as 2g/l, 3μg/l and IU/ml respectively. Eventually the product produced was analyzed qualitatively by thin layer chromatography and quantitatively by standard ninhydrin method. The product purity was confirmed by Fourier transform infrared spectroscopy and proton nuclear magnetic resonance spectroscopy. Finally the fermented broth was subjected to crystallization at the isoelectric point (pH3.2) of L-glutamic acid using IN HCl.